Question 1

The specimen preparation that is best for viewing cell motility is

Accepted Answer

A)  hanging drop.
B)  fixed-stained smear.
C)  Gram stain.
D)  negative stain.
E)  flagellar stain.F) A) and D)
G) None of the above

Question 2

A bacterial species that grows on blood agar but will not grow on trypticase soy agar is termed an anaerobe.

Accepted Answer

A) True 
 B)False

Question 3

Each of the following are characteristics of microorganisms that pose unique challenges to their study EXCEPT

Accepted Answer

A)  the fact that they must be viewed through a microscope rather than the unaided eye.
B)  the fact that many do not have membrane-enclosed nucleus or other organelles.
C)  the fact that they often exist in complex communities and individual species may be difficult to isolate from others.
D)  the fact that they usually must be grown and studied under somewhat artificial conditions in vitro rather than in their natural habit.E) All of the above
F) A) and B)

Question 4

To prepare large volumes of influenza virus for use in the flu vaccines each year, the virus is grown in embryonated hen eggs.This is an example of the use of

Accepted Answer

A)  enriched media.
B)  selective media.
C)  live media.
D)  complex media.E) A) and B)
F) A) and C)

Question 5

Which of the following is essential for development of discrete, isolated colonies?

Accepted Answer

A)  broth medium
B)  differential medium
C)  selective medium
D)  solid medium
E)  assay mediumF) A) and E)
G) C) and E)

Question 6

In lab, Tom was given a mixed culture.His objective is to isolate single colonies.What should be used to accomplish this goal?

Accepted Answer

A)  sterile swab, loop dilution, TSB
B)  hockey stick, spread plate technique, and 4 degree Celsius incubation temperature
C)  loop dilution, TSA, hockey stick, 37 degree Celsius incubator
D)  inoculating loop, incinerator, streak plate method, 37 degree Celsius incubator
E)  sterile swab, streak plate method, 37 degree Celsius incubatorF) A) and E)
G) D) and E)

Question 7

The _____ of the microscope holds and allows selection of the objective lenses.

Accepted Answer

The answer of The _____ of the microscope holds and...

Question 8

Which microscope is the most widely used to show stained cells against a bright background?

Accepted Answer

A)  bright-field
B)  dark-field
C)  phase-contrast
D)  fluorescence
E)  electronF) B) and E)
G) A) and D)

Question 9

Identification relies entirely on biochemical test results.

Accepted Answer

A) True 
 B)False

Question 10

A pure culture contains only

Accepted Answer

A)  one species of microorganism.
B)  bacteria.
C)  a variety of microbes from one source.
D)  All of these choices are correct.E) A) and B)
F) A) and C)

Question 11

Which of the following magnifies the specimen to produce the real image of the specimen?

Accepted Answer

A)  condenser
B)  objective lens
C)  ocular lens
D)  body
E)  nosepieceF) B) and D)
G) A) and B)

Question 12

_____ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the slide to the objective lens.

Accepted Answer

The answer of _____ has the same optical qualities as...

Question 13

Bacteria that require special growth factors and complex organic substances are called

Accepted Answer

A)  fastidious.
B)  pathogenic.
C)  harmless.
D)  anaerobic.
E)  aerobic.F) A) and B)
G) A) and C)

Question 14

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Accepted Answer

A)  differential
B)  selective
C)  enumeration
D)  enriched
E)  reducingF) B) and E)
G) A) and C)

Question 15

Viruses, Rickettsia, and Chlamydia are described as obligate intracellular parasites because they can only be propagated inside living host cells.Which of the following would be appropriate for their cultivation?

Accepted Answer

A)  enriched media
B)  cell or tissue cultures
C)  media that contains sheep red blood cells
D)  differential mediaE) None of the above
F) A) and C)

Question 16

Why is immersion oil often used when viewing specimens under the microscope?

Accepted Answer

A)  to stain the cells blue
B)  to prevent the smear from drying out
C)  to slow bacterial movement so you can see cells better
D)  to increase the resolution
E)  to reduce the amount of heat reaching the slide from the light sourceF) B) and C)
G) None of the above

Question 17

You are doing research on a bacterial species, trying to determine the nature and structure of a number of intracellular inclusions.Which type of microscopy would provide the best view of these intracellular structures?

Accepted Answer

A)  scanning electron microscopy
B)  transmission electron microscopy
C)  bright-field microscopy
D)  dark-Field microscopyE) A) and B)
F) A) and C)

Question 18

Which is incorrect about chocolate agar?

Accepted Answer

A)  It can be used to cultivate Neisseria.
B)  It usually uses sheep blood that has been heated.
C)  It has chocolate extract in it.
D)  It is an enriched medium.
E)  It is used to grow fastidious bacteria.F) A) and B)
G) B) and E)

Question 19

The Six "I's" of studying microorganisms include all of the following except

Accepted Answer

A)  inoculation.
B)  incubation.
C)  infection.
D)  isolation.
E)  identification.F) A) and D)
G) C) and D)

Question 20

Media that contains extracts from plants, animals, or yeasts are

Accepted Answer

A)  synthetic.
B)  complex.
C)  reducing.
D)  enriched.
E)  All of these choices are correct.F) B) and D)
G) B) and C)

Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

The specimen preparation that is best for viewing cell motility is

Correct AnswerverifiedShow Answer

The Six "I's" of studying microorganisms include all of the following except

Correct AnswerverifiedShow Answer

To prepare large volumes of influenza virus for use in the flu vaccines each year, the virus is grown in embryonated hen eggs.This is an example of the use of

Correct AnswerverifiedShow Answer

Why is immersion oil often used when viewing specimens under the microscope?

Correct AnswerverifiedShow Answer

Viruses, Rickettsia, and Chlamydia are described as obligate intracellular parasites because they can only be propagated inside living host cells.Which of the following would be appropriate for their cultivation?

Correct AnswerverifiedShow Answer

The _____ of the microscope holds and allows selection of the objective lenses.

Correct AnswerverifiedShow Answer

Bacteria that require special growth factors and complex organic substances are called

Correct AnswerverifiedShow Answer

Media that contains extracts from plants, animals, or yeasts are

Correct AnswerverifiedShow Answer

A bacterial species that grows on blood agar but will not grow on trypticase soy agar is termed an anaerobe.

Correct AnswerverifiedShow Answer

Which of the following magnifies the specimen to produce the real image of the specimen?

Correct AnswerverifiedShow Answer

Which of the following is essential for development of discrete, isolated colonies?

Correct AnswerverifiedShow Answer

Which is incorrect about chocolate agar?

Correct AnswerverifiedShow Answer

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Correct AnswerverifiedShow Answer

Each of the following are characteristics of microorganisms that pose unique challenges to their study EXCEPT

Correct AnswerverifiedShow Answer

_____ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the slide to the objective lens.

Correct AnswerverifiedShow Answer

Identification relies entirely on biochemical test results.

Correct AnswerverifiedShow Answer

You are doing research on a bacterial species, trying to determine the nature and structure of a number of intracellular inclusions.Which type of microscopy would provide the best view of these intracellular structures?

Correct AnswerverifiedShow Answer

In lab, Tom was given a mixed culture.His objective is to isolate single colonies.What should be used to accomplish this goal?

Correct AnswerverifiedShow Answer

A pure culture contains only

Correct AnswerverifiedShow Answer

Which microscope is the most widely used to show stained cells against a bright background?

Correct AnswerverifiedShow Answer

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